Title: assistant professor (Dr./PhD)
Association/Company: Wagenigen University
Sarah D’Adamo studied (MSc. and PhD) at the University of Padova, IT. After a post-doctoral period in USA, she has been lead scientist at Algenuity, a microalgal biotech company in UK. From 2018 she is a tenure Assistant Professor in microalgae cell factory design at the Bioprocess Engineering group of Wageningen University, NL. Overall she has >10 years’ of international academic, industrial experience in the field of microalgae physiology and biotechnology. Major interests: microalgae strain engineering, synthetic biology, nutraceutical production, bioprocess development.
Obtaining more from microalgae
CRISPR-CAS9 and microalgae
Microalgae have caught industrial interest as promising photosynthetic production platforms for several high-value compounds such as fatty acids, hydrocarbons, pigments, and polysaccharides. Although, in order to become an economically feasible chassis, these microorganisms must be optimized in solar energy conversion, carbon capture and utilization, and the partitioning of metabolic fluxes 1. With the recent progress in biotechnology and synthetic biology, tools and techniques for the genetic manipulation and isolation of microalgae are expanding quickly. Here we report our recent progress aimed at providing novel and faster tools for microalgal biotechnology, by using genome editing techniques (CRISPR-Cas), E. coli conjugation methods, FACS for fast screening of cells with desired characteristics 2–4.
1. Work VH, D’Adamo S, Radakovits R, Jinkerson RE, Posewitz MC. Improving photosynthesis and metabolic networks for the competitive production of phototroph-derived biofuels. Curr Opin Biotechnol. 2012;23(3). doi:10.1016/j.copbio.2011.11.022
2. Südfeld C, Hubáček M, Figueiredo D, et al. High-throughput insertional mutagenesis reveals novel targets for enhancing lipid accumulation in Nannochloropsis oceanica. Metab Eng. 2021;66(May):239-258. doi:10.1016/j.ymben.2021.04.012
3. Südfeld C, Hub M, Adamo SD, Barbosa MJ. Optimization of high-throughput lipid screening of the microalga Nannochloropsis oceanica using BODIPY 505 / 515. 2020;(June). doi:10.1016/j.algal.2020.102138 4. Naduthodi MIS, Südfeld C, Avitzigiannis EK, et al. Comprehensive Genome Engineering Toolbox for Microalgae Nannochloropsis oceanica Based on CRISPR-Cas Systems. ACS Synth Biol. 2021;10(12):3369-3378. doi:10.1021/acssynbio.1c00329